The Mona Lisa at one point had eyebrows and eyelashes according to the analysis of a 240 megapixel scan done by a Parisian engineer who has studied the painting for more than 3,000 hours. – WTF Fun Facts
Source: https://www.telegraph.co.uk/culture/art/3668700/Solved-Why-Mona-Lisa-doesnt-have-eyebrows.html